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The film explores the adventure of four girls who start their lives again by participating in the illegal underground murder 'Blood Girl'. That dangerous experiment was held in an abandoned school building in Roppongi every night as these girls were making more conflicts in that game. Satsuki (Yuria Haga) is a girl with sex identity disorder, and Chinatsu (Asami Tada) is a woman who had a bad husband, Miku (Ayami Misaki) is the queen of S&M and Mayo (Reina Koike) is a different girl with a lolita face.